HBsAg阳性孕产妇miR-122 rs7227488基因多态性与HBV宫内感染发生的相关性

肝脏 ›› 2020, Vol. 25 ›› Issue (10): 1034-1037.

HBsAg阳性孕产妇miR-122 rs7227488基因多态性与HBV宫内感染发生的相关性

林婧, 夏霖, 冯地路

430000 武汉华中科技大学附属协和医院西院妇产科

收稿日期:2020-04-08 出版日期:2020-10-31 发布日期:2020-12-18
基金资助:
国家自然科学基金青年科学基金(81601260)

Correlation between miR-122 rs7227488 gene polymorphism and HBV intrauterine infection in HBsAg-positive pregnant women

LIN Jing, XIA Lin, FENG Di-lu

Department of Obstetrics and Gynecology, Xiyuan Hospital of Union Hospital affiliated to Huazhong University of science and technology, Wuhan 430000, China

Received:2020-04-08 Online:2020-10-31 Published:2020-12-18

摘要/Abstract

摘要： 目的探讨HBsAg阳性孕产妇miR-122启动子基因多态性与乙型肝炎病毒(HBV)宫内感染易感性的关系。方法选取2017年10月至2019年7月华中科技大学附属协和医院西院妇产科行产前检查并分娩的141对HBsAg阳性孕妇及新生儿为研究对象,收集孕妇一般资料(年龄、分娩时孕周、分娩方式、新生儿性别等),将HBsAg阳性孕妇分为HBV宫内感染组47例,无HBV宫内感染组94例,运用聚合酶链反应-限制性片段长度多态性(PCR-RELP)检测研究对象miR-122启动子rs7227488基因多态性,分析miR-122启动子rs7227488基因多态性与新生儿HBV感染的相关性,采用logistic回归分析影响HBsAg阳性孕产妇发生HBV宫内感染发生的危险因素。结果 Hardy-Weinberg平衡定律显示,无HBV宫内感染组与HBV宫内感染组G/A各基因型符合Hardy-Weinberg平衡定律(均P>0.05);与无HBV宫内感染组44.6%相比,HBV宫内感染组55.3% rs7227488位点G/A点突变率显著升高(P<0.05);二元logistic回归分析显示,HBV DNA含量、miR-122 基因多态性位点rs7227488 AA型是HBsAg阳性孕产妇的HBV宫内感染的危险因素(OR:1.512、1.898,均P<0.05)。结论 HBsAg阳性孕产妇miR-122 rs7227488 AA基因型与HBV宫内感染的发生密切相关。

关键词: 乙型肝炎病毒, 微小RNA-122, 基因多态性, 宫内感染

Abstract: Objective To investigate the relationship between rs7227488 polymorphism site at the gene promoter region of miR-122 and the susceptibility of intrauterine infection in HBsAg-positive pregnant women. Methods One hundred and fourty-one HBsAg positive pregnant women who underwent prenatal examination and deliveried were selected in this study. General data of the pregnant women (age, gestational week at delivery, mode of delivery, sex of newborn) were collected. The HBsAg-positive pregnant women were divided into HBV intrauterine infection group (47 cases) and non-HBV intrauterine infection group (94 cases) according to the occurance of HBV infection in their neonates. The rs 7227488 polymorphism of miR 122 gene in all subjects was detected using polymerase chain reaction-restriction fragment length polymorphism (PCR-RELP). Risk factors of HBV intrauterine infection in HBsAg positive pregnant women and the correlation between rs7227488 and neonatal HBV infection were analyzed by logistic regression analysis. Results The G/A genotypes of rs7227488 in non-HBV intrauterine infection group and HBV intrauterine infection group were accordant with Hardy-Weinberg equilibrium law (P>0.05). The G/A mutation rate of rs7227488 in non-HBV intrauterine infection group (44.6%) was significantly higher than that of the HBV intrauterine infected group (55.3%) (P<0.05). Bivariate logistic regression analysis showed that HBV DNA load and rs7227488 AA type were risk factors for intrauterine HBV infection in HBsAg-positive pregnant women (Or=1.512,1.898,all P<0.05). Conclusion The miR-122 rs7227488 AA genotype of HBsAg-positive pregnant and lying-in women are risk factors of intrauterine HBV infection.

Key words: Hepatitis B virus, MicroRNA-122, Gene polymorphism, Intrauterine infection

林婧, 夏霖, 冯地路. HBsAg阳性孕产妇miR-122 rs7227488基因多态性与HBV宫内感染发生的相关性[J]. 肝脏, 2020, 25(10): 1034-1037.

LIN Jing, XIA Lin, FENG Di-lu. Correlation between miR-122 rs7227488 gene polymorphism and HBV intrauterine infection in HBsAg-positive pregnant women[J]. Chinese Hepatolgy, 2020, 25(10): 1034-1037.

参考文献 12

[1]	张磊, 邵中军. HBV宫内传播研究在乙型肝炎防控工作中发挥着重要的作用. 中华流行病学杂志,2019,40:1055-1058.
[2]	邹怀宾, 赵梦鱼, 陈煜, 等. HBsAg阳性母亲所分娩婴儿完成主被动免疫后的免疫保护效果及其持久性研究. 北京医学, 2019, 41:888-891.
[3]	Amadorcañizares Y, Bernier A, Wilson JA, et al.miR-122 does not impact recognition of the HCV genome by innate sensors of RNA but rather protects the 5' end from the cellular PyroPhosPhatases, DOM3Z and DUSP11. Nucleic Acids Res, 2018, 46:5139-5158.
[4]	宋晓菲, 徐元宏. 丙型肝炎病毒感染患者血清外泌体miR-122的表达量及临床意义探讨. 安徽医科大学学报, 2019, 54:131-134.
[5]	Lu X, Liu Y, Xuan W, et al.Circ1639 induces cells inflammation responses by sponging miR-122 and regulating TNFRSF13C expression in alcoholic liver disease. Toxicol Lett, 2019, 314:89-97.
[6]	Fathi-Kazerooni M, Kazemnejad S, Khanjani S, et al.Down-regulation of miR-122 after transplantation of mesenchymal stem cells in acute liver failure in mice model. Biologicals, 2019, 58:64-72.
[7]	Fournier C, Hoffmann TW, Morel V, et al.Claudin-1, miR-122 and apolipoprotein E transductions improve the permissivity of SNU-182, SNU-398 and SNU-449 hepatoma cells to hepatitis C virus. J Viral Hepat, 2018, 25:63-71.
[8]	张轩, 王淏. 氧化苦参碱对miR-122低表达、感染HBV肝细胞的抗病毒作用观察. 山东医药, 2018, 58:31-33.
[9]	荆晓晴, 姚丽, 谢妍, 等. 乙肝病毒携带者外周血单核细胞miR-122表达与病毒增殖的关系研究. 病毒学报, 2019, 35:895-899.
[10]	熊玉娟, 刘颖异, 庄家玲. miR-122启动子rs7227488单核苷酸多态性及其对HCV易感性研究. 中国输血杂志, 2018, 31:727-731.
[11]	Caviglia GP, Abate ML, Gaia S, et al.Risk of hepatocellular carcinoma in HBV cirrhotic patients assessed by the combination of miR-122, AFP and PIVKA-II. Panminerva Med, 2017, 59:283-289.
[12]	van der Ree MH, Jansen L, Kruize Z, et al. Plasma microRNA levels are associated with HBeAg status and treatment response in chronic hepatitis B patients. J Infect Dis, 2017, 215:1421-1429.

[1]	邵曙光, 孙海霞, 冯柳芳. 隐匿性HBV感染患者HBV C区变异的临床意义[J]. 肝脏, 2020, 25(8): 857-859.
[2]	王骁, 周锡建, 顾培. 精确放疗治疗原发性肝癌后HBV再激活的影响因素研究[J]. 肝脏, 2020, 25(7): 702-704.
[3]	马皖苏, 赵林, 李红生. CLIF-SOFA评分对HBV-ACLF患者器官衰竭的评估价值[J]. 肝脏, 2020, 25(6): 578-581.
[4]	周培, 渠淑云. 替诺福韦阻断高HBV DNA载量孕妇母婴传播临床效果观察[J]. 肝脏, 2020, 25(4): 393-395.
[5]	李永涛, 卜贤玉, 李滨. 替诺福韦酯补救治疗对HBV感染肝硬化患者的疗效分析[J]. 肝脏, 2020, 25(4): 400-402.
[6]	黄珊珊, 赵静雅, 陈洁, 杨扬. 前列腺素E2促进乙型肝炎病毒复制的机制研究[J]. 肝脏, 2020, 25(3): 277-281.
[7]	王贤, 林涛发, 詹志瑜, 王少扬. 不同免疫状态慢性HBV感染患者的T淋巴细胞及NKG2D表达差异研究[J]. 肝脏, 2020, 25(2): 131-134.
[8]	龙云铸, 贺萧瑾, 李丹, 周娟, 周青, 谭英征. HBx介导SOCS-1基因甲基化致肝细胞癌发生的机制[J]. 肝脏, 2020, 25(2): 202-205.
[9]	刘娇, 刘青, 王大明, 贾兴旺. 慢性乙型肝炎患者血清外泌体mir-122、mir-146a表达与HBV DNA载量的相关性[J]. 肝脏, 2020, 25(10): 1038-1042.
[10]	张莉, 孟现民, 董平. 上海市公共卫生临床中心2015—2019年乙型肝炎病毒基因突变分析[J]. 肝脏, 2020, 25(10): 1043-1047.
[11]	李丽, 单奔, 潘修成, 傅涓涓. 慢性乙型肝炎患者血清维生素D水平的临床意义[J]. 肝脏, 2020, 25(10): 1048-1051.
[12]	王欣茹, 肖丽, 杨莉莉, 张会, 汤小霞, 孙鸿展, 咸建春. 不同年龄孕妇HBV感染现状的血清流行病学分析[J]. 肝脏, 2020, 25(1): 76-77.
[13]	谭文敏, 赖庭文, 韩锋. IL-28B基因多态性对直接抗病毒药物治疗慢性丙型肝炎患者疗效的预测价值[J]. 肝脏, 2019, 24(7): 776-779.
[14]	钟慧筠, 殷思纯. HBV相关慢加急性肝衰竭器官衰竭危险因素及短期预后分析[J]. 肝脏, 2019, 24(6): 658-661.
[15]	华燕美. 两种乙型肝炎病毒核酸定量检测试剂的检验重复性与一致性比较[J]. 肝脏, 2019, 24(6): 692-694.