关键词: 不明原因肝病, 生物样本库, 信息化管理, 质量控制

Abstract: Objective To explore the construction method of biobank, sample data management and quality control of stored samples.Methods Plasma, buffy coat and peripheral blood mononuclear cells (PBMC) samples from patients who were diagnosed as unknown etiology liver disease during July 2018 and June 2019 in the Second Hospital of Nanjing, Nanjing University of Chinese Medicine were harvested, coded and stored in -80 ℃ refrigerator or liquid nitrogen. The full-time staff of the biobank log in the system of Nanjing multi center biological sample database to input the clinical information and sample coding information of the donors. Samples were randomly selected for the extraction and identification of DNA and RNA. Results Up to June 2019, a total of 2 007 samples were collected, including 1 625 plasma samples, 333 buffy coat samples and 49 PBMCs samples. In addition, the quality of DNA and RNA of 5 buffy coat samples were detected. All random samples were in accordance with the quality standards of samples and could meet the requirements of the following studies.Conclusion A biobank of unknown etiology liver disease and standardized biobank operation and management procedures were established, which can provide experimental samples and detailed clinical data for further study of unknown etiology liver disease.

Key words: Unknown etiology liver disease, Biobank, Information management, Quality control