关键词: 非酒精性脂肪性肝病, 脂质组学, 肥胖

Abstract: Objective To compare the serum lipid profiles between non obese and obese patients with non-alcoholic fatty liver disease (NAFLD) patients. Methods A total of 60 NAFLD patients were recruited and categorized into two groups based on body mass index(BMI): non-obese (BMI<25 kg/m²) and obese (BMI ≥ 25 kg/m²) . Serum samples from all patients were analyzed using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS) for lipidomics profiling. Differential lipids were identified through multiple statistical methods, including fold change anaysis, partial least squares discriminant analysis (PLS-DA), and orthogonal partial least squares discriminant analysis (OPLS-DA). Permutation tests were conducted to validate the results. Results A total of 90 differential lipids were identified, Including sphingomyelin (SM), Ether linked phosphatidylcholine (EtherPC), hexosylceramides (HexCer), and phosphatidylethanolamine(PC). KEGG pathway enrichment analysis revealed significant alterations in several metabolic pathways in non-obese NAFLD patients, such as the sphingolipid signaling pathway, necroptosis, glycerophospholipid metabolism pathway, glycosylphosphatidylinositol biosynthetic pathway, and tumor choline metabolism pathway. Conclusion Significant differences in serum lipid mass spectrometry profiles were observed between non-obese and obese NAFLD patients, particularly in PC, PE, SM levels.

Key words: Non-alcoholic fatty liver disease, Lipidomics, Obese