MiR-152过表达对对乙酰氨基酚诱导的急性肝损伤的保护机制

肝脏 ›› 2019, Vol. 24 ›› Issue (2): 147-149.

MiR-152过表达对对乙酰氨基酚诱导的急性肝损伤的保护机制

张豪洁, 林琛琛

264002 山东烟台解放军第一○七医院肝胆外科(张豪洁),麻醉科(林琛琛)

收稿日期:2018-09-11 发布日期:2020-04-10
通讯作者: 林琛琛,Email:1262510724@qq.com

Protective mechanism of miR-152 overexpression on acetaminophen-induced acute liver injury

ZHANG Hao-jie, LIN Chen-chen

Department of Hepatobiliary surgery, 107 Hospital of Chinese People′s Liberation Army, Yantai, Shandong 264002, China(ZHANG Hao-jie); Department of anesthesiology, 107 Hospital of Chinese People′s Liberation Army, Yantai, Shandong 264002, China(LIN Chen-chen)

Received:2018-09-11 Published:2020-04-10

摘要/Abstract

摘要： 目的探讨microRNA-152(miR-152)在对乙酰氨基酚(APAP)诱导的急性肝损伤发病机制中的作用。方法 B6小鼠腹腔注射APAP诱导急性肝损伤。在miR-152干预实验中,在腹腔注射APAP前24 h给予miR-152 激动剂、拮抗剂以及相应的阴性对照。评估APAP诱导的肝损伤的程度;实时荧光定量PCR测量miR-152及其下游调节物的表达水平。结果与对照组相比,APAP处理后诱导miR-152表达增高2倍(P<0.05)。与对照组相比,miR-152在体内过表达明显降低ALT(8295±557.1 比 3995±551.9) U/L, (P<0.05)、AST(8065±1057 比 3623±548.4) U/L, (P<0.05)水平,但miR-152拮抗剂明显增加了ALT(6973±649.6 比 11915±555.5) U/L,(P<0.05)、AST(6130±566.7 比 9415±622.0) U/L, (P<0.05)水平。结论 miR-152可抑制APAP诱导的急性肝损伤。

关键词: 对乙酰氨基酚, miR-152, 急性肝损伤

Abstract: Objective Overdose of acetaminophen (APAP) clinically could result in severe and fatal liver failure.The aim of this study was to investigate the molecular role of micro ribonucleic acid-152 (miR-152) in the pathogenesis of APAP-induced acute liver injury.Methods B6 mice were intraperitoneally injected with APAP to induce acute liver injury. In the experiment, mice were given miR-152 agomir, antagomir and corresponding negative controls 24 hours before APAP induction, respectively. Assays of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and histology were conducted to evaluate the degree of APAP-induced liver injury. The expression levels of miR-152 and inflammatory factors were measured using quantitative real time polymerase chain reaction.Results Compared with the control group, the expression level of miR-152 increased by 2 fold after induction of APAP treatment (P< 0.05). The overexpression of miR-152 in vivo alleviated acute liver injury caused by APAP overdose, showing lower levels of ALT (8295 ± 557.1 vs. 3995 ± 551.9, P< 0.05) and AST (8065 ± 1057 vs. 3623 ± 548.4, P< 0.05). However, silencing miR-152 had a detrimental effect during the injury process, which were with higher levels of ALT (6973 ± 649.6 vs. 11915 ± 555.5, P< 0.05) and AST (6130 ± 566.7 vs. 9415 ± 622.0, P< 0.05).Conclusion It was demonstrated that miR-152 alleviated APAP-induced acute liver injury, which suggesting potential therapeutic value of miR-152 mimics on treatment of this disorder clinically.

Key words: Acetaminophen, Micro ribonucleic acid-152, Acute liver injury

张豪洁, 林琛琛. MiR-152过表达对对乙酰氨基酚诱导的急性肝损伤的保护机制[J]. 肝脏, 2019, 24(2): 147-149.

ZHANG Hao-jie, LIN Chen-chen. Protective mechanism of miR-152 overexpression on acetaminophen-induced acute liver injury[J]. Chinese Hepatolgy, 2019, 24(2): 147-149.

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