RNA、lnc-EGFR对肝细胞癌免疫逃避调节的相关性

摘要/Abstract

摘要： 目的探讨RNA、表皮生长因子受体长链非编码RNA(lnc-EGFR)对肝细胞癌(HCC)免疫逃避调节的相关性。方法通过医院病理档案室随机选择保定市第一中心病医院2017年1月至2019年1月收集的HCC标本30份和癌旁正常组织30份,采用RT-PCR法检测各组织中RNA、lnc-EGFR表达水平;蛋白质印迹法检测HCC组织中化学因子和细胞免疫因子表达情况,并分析RNA、lnc-EGFR与其相关性;将HCC细胞株随机分为两组,分别为RNA、lnc-EGFR抑制组(n=15)与空白对照组(n=15),MTT法检测细胞增殖情况,流式细胞仪检测细胞凋亡情况与细胞周期分布情况。结果癌旁正常组织中RNA、lnc-EGFR表达水平分别为(0.46±0.12)和(0.62±0.15)低于HCC组织(0.22±0.06)和(0.14±0.05) (P<0.05)。HCC组织中CXCL12-CXCR4表达水平低于癌旁正常组织(P<0.05);HCC组织中CCL20-CCR6、CX3CLI-CX3CR1高于癌旁正常组织(P<0.05);HCC组织中细胞免疫因子IL-6、IL-10、TGF-β、TNF-α表达水平高于癌旁正常组织(P<0.05);RNA、lnc-EGFR表达水平与CXCL12-CXCR4呈显著负相关(P<0.05),与CCL20-CCR6、CX3CLI-CX3CR1、IL-6、IL-10、TGF-β、TNF-α呈显著正相关关系(P<0.05);HCC、癌旁正常组织中细胞相对吸光度、细胞凋亡率、细胞周期占比均具有统计学差异(P<0.05)。结论 RNA、lnc-EGFR在HCC组织中呈高表达水平,可通过上调组织中化学因子和细胞免疫因子水平来提高癌细胞免疫逃避调节水平,与HCC免疫逃避调节具有显著相关性。

关键词: RNA, lnc-EGFR, 肝细胞癌, 免疫逃避调节, 相关性

Abstract: Objective To investigate the role of ribonucleic acid (RNA) and long non-coding RNA of epidermal growth factor receptor (lnc-EGFR) in hepatocellular carcinoma (HCC) immune evasion. Methods A total of 30 HCC specimens and 30 normal adjacent tissues collected in our hospital from January 2017 to January 2019 were randomly selected. The expression levels of RNA and lnc-EGFR in tissues were detected by reverse transcription polymerase chain reaction, and the expression of chemical factors and cellular immune factors in HCC tissues were detected by Western blot. HCC cell lines were randomly divided into 2 groups: RNA/lnc-EGFR inhibition group (n=15) and blank control group (n=15). The 3-(4, 5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide assay was used to detect cell proliferation, and flow cytometry was used to detect cell apoptosis and cell cycle distribution. Results The expression levels of RNA and lnc-EGFR in adjacent normal tissues (0.46 ± 0.12, 0.62 ± 0.15) were lower than those in HCC tissues (0.22 ± 0.06, 0.14 ± 0.05) (P<0.05). The expression level of C-X-C motif chemokine ligand 12 (CXCL12)-C-X-C motif chemokine receptor 4 (CXCR4) in HCC tissues was lower than that in adjacent normal tissues (P<0.05). The expression levels of C-C motif chemokine ligand 20 (CCL20)-C-C motif chemokine receptor 6 (CCR6) and C?X3?C motif chemokine ligand 1 (CX3CL1)-C?X3?C motif chemokine receptor 1 (CX3CR1) in HCC tissues were higher than those in adjacent normal tissues (P<0.05). The expression levels of cellular immune factors including interleukin-6 (IL-6), interleukin-10 (IL-10), transforming growth factor-β (TGF-β) and tumor necrosis factor-α (TNF-α) in HCC tissues were higher than those in adjacent normal tissues (P<0.05). The expression levels of RNA and lnc-EGFR were significantly negatively correlated with CXCL12-CXCR4 (P<0.05), and significantly positively correlated with CCL20-CCR6, CX3CL1-CX3CR1, IL-6, IL-10, TGF-β and TNF-α (P<0.05); The relative absorbance, apoptosis rate and cell cycle ratio of HCC and adjacent normal tissues were statistically different (P<0.05). Conclusion RNA and lnc-EGFR are highly expressed in HCC tissues, and can upregulate immune evasion of cancer cells by up-regulating the levels of chemical factors and cellular immune factors in tissues, which has a significant correlation with HCC immune evasion regulation.

Key words: Ribonucleic acid, long non-coding ribonucleic acid of epidermal growth factor receptor, Hepatocellular carcinoma, Immune evasion, Correlation

魏媛媛, 田艳红, 李红静, 张浩, 李木松. RNA、lnc-EGFR对肝细胞癌免疫逃避调节的相关性[J]. 肝脏, 2020, 25(2): 142-145.

WEi Yuan-yuan, TIAN Yan-hong, Li Hong-jing, ZHANG Hao, LI Mu-song. Role of RNA and lnc-EGFR in hepatocellular carcinoma immune evasion[J]. Chinese Hepatolgy, 2020, 25(2): 142-145.

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