肝癌患者肠道微生态结构变化的特点分析

摘要/Abstract

摘要： 目的探讨原发性肝癌患者肠道菌群结构的变化。方法选择2017年9月至2018年12月广东省第二人民医院诊疗的原发性肝癌患者87例,另取健康体检者80名作为健康对照。制备微生物基因组DNA后 Illumina平台进行高通量测序。结果原发性肝癌组和健康对照组间α多样性差异无统计学意义(P>0.05)。肝癌组拟杆菌门和变形菌门所占百分比为(56.41±4.63)%和(9.26±1.82)%,显著高于健康对照组的(53.32±4.22)%和(7.42±1.16)%(均P<0.05)。肝癌组厚壁菌门和放线菌门所占百分比为(32.62±3.75)%和(0.34±0.05)%,显著低于健康对照组的(37.25±4.13)%和(0.62±0.11)%(均P<0.05)。肝癌组拟杆菌属和大肠杆菌螺旋杆菌属所占百分比为(50.83±4.15)%和(11.35±1.87)%,显著高于健康对照组的(42.45±3.84)%和(8.52±1.71)%(均P<0.01)。肝癌组双歧杆菌属和梭菌属所占百分比为(21.13±3.64)%和(10.44±1.25)%,显著低于健康对照组的(28.54±4.13)%和(14.28±1.52)%(均P<0.01)。结论肝癌患者肠道微生态系统中拟杆菌以及其隶属的拟杆菌门,大肠杆菌螺旋杆菌属属以及其隶属的变形菌门所占比例明显著高于对照组,双歧杆菌属以及其隶属的放线菌门和梭菌属以及其隶属的厚壁菌门所占比例明显著低于对照组。

关键词: 原发性肝癌, 肠道微生态系统, 高通量测序

Abstract: Objective To detect the characteristics of intestinal microecological structure changes in patients with hepatocellular carcinoma.Methods 87 patients with hepatocellular carcinoma were enrolled in this study. Another 80 healthy persons were taken as control. The microbial genomic was prepared for high throughput sequencing by Illumina platform. Results There was no significant difference in α diversity between hepatocellular carcinoma group and control group (P>0.05). The percentage of bacteroidetes and proteobacteria in hepatocellular carcinoma group was 56.41±4.63% and 9.26±1.82%, which was significant higher than that of 53.32±4.22% and 7.42±1.16% in control group(P<0.05 and P<0.01). The percentage of firmicutes and actinobacteria in hepatocellular carcinoma group was 32.62±3.75% and 0.34±0.05%, which was significant lower than that of 37.25±4.13% and 0.62±0.11% in control group(P<0.05 and P<0.01). The percentage of bacteroides and H.hepaticus in hepatocellular carcinoma group was 50.83±4.15% and 11.35±1.87%, which was significant higher than that of 42.45±3.84% and 8.52±1.71% in control group(P<0.01). The percentage of bifidobacterium and clostridium in hepatocellular carcinoma group was 21.13±3.64% and 10.44±1.25%, which was significant lower than that of 28.54±4.13% and 14.28±1.52% in control group(P<0.01).Conclusion The proportion of bacteroidetes, proteobacteria, bacteroides, and H.hepaticus in patients with hepatocellular carcinoma was significantly higher than that of control group. The proportion of firmicutes, actinobacteria, bifidobacterium and clostridium in patients with hepatocellular carcinoma was significantly lower than that of control group.

Key words: hepatocellular carcinoma, intestinal microecosystem, Illumina genome analyzer

孙亮, 周旋, 刘桂治, 林茂锐, 李明友, 曹东林. 肝癌患者肠道微生态结构变化的特点分析[J]. 肝脏, 2020, 25(3): 267-269.

SUN Liang, ZHOU Xuan, LIU Gui-zhi, LIN Mao-rui, LI Ming-you, CAO Dong-lin.. Characteristics of intestinal microecological structure changes in patients with hepatocellular carcinoma[J]. Chinese Hepatolgy, 2020, 25(3): 267-269.

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