The correlation between serum soluble E-cadherin expression and oxidative stress in patients with chronic hepatitis B

Abstract

Abstract: Objective To investigate the correlation between serum soluble E-cadherin expression and oxidative stress in patients with chronic hepatitis B.Methods Fifty-four HBsAg-positive patients that were diagnosed as HBV infection were selected in this study. They were divided into mild (16 cases), moderate (20 cases) and severe (18 cases) subgroups according to the severity of CHB. Twenty healthy subjects were also enrolled as the control group. The serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), sE-cadherin, glutathione (GSH), Malondialdehyde (MDA), NADPH oxidase 2 (NOX2), superoxide dismutase (SOD) and HBV DNA load were detected. The data were applied to Pearson correlation analysis. The influencing factors for the severity of CHB were analyzed by multivariate binary logistic regression.Results Compared with the control group, the serum levels of AST, ALT, sE-cadherin, MDA, NOX2 and HBV DNA were significantly increased [AST: (72.59±10.42), (107.59±8.24), (160.13±24.84) vs (28.51±4.67) U/L, ALT: (64.27±8.91), (118.43±15.37), (194.30±12.61) vs (24.18±5.64) U/L, sE-cadherin: (566.27±73.16), (671.51±80.43), (735.65±43.29) vs (309.51±43.28) pg/mL, MDA: (12.58±2.37), (17.35 ±3.51), (24.35±4.18) vs (7.59±0.83) nmol/mL, NOX2: (1837.25±106.25), (2766.52±428.14), (2766.52±428.14) vs (972.58±34.82) pg/mL, and HBV DNA: (42.27±8.37), (76.26±15.52), (101.64±8.94)log₁₀ copies/mL, On the contrary, the levels of GSH and SOD significantly decreased [GSH: (9.92±1.58), (5.23±0.94), (3.77±0.51) vs (15.16±2.26) ng/mL, SOD: (140.75±15.26), (127.31±10.58), (97.35±10.94) vs (168.29±24.62) U/mL] in each CHB group, and changed accordingly with the aggravation of the disease (P<0.05). The serum levels of sE-cadherin were positively correlated with AST, ALT, HBV DNA, MDA and NOX2 (r=0.591, 0.538, 0.675, 0.527, 458, respectively, P<0.05), whereas negatively correlated with GSH and SOD (r=-0.499, -0.492, P<0.05). The result of multivariate analysis showed that sE-cadherin, HBV DNA and NOX2 were the influencing factors for severe CHB (P<0.05).Conclusion Serum sE-cadherin in patients with chronic hepatitis B viral infection increased with the severity of the disease, which was positively associated with the oxidative stress in the patients.

Key words: Chronic hepatitis B, Disease severity, Soluble E-cadherin, Oxidative stress

XU Jun, XIE Guo-yan, HUANG Min. The correlation between serum soluble E-cadherin expression and oxidative stress in patients with chronic hepatitis B[J]. Chinese Hepatolgy, 2021, 26(1): 19-21.

References

[1] Cai J, Han T, Nie C, et al. Biomarkers of oxidation stress, inflammation, necrosis and apoptosis are associated with hepatitis B-related acute-on-chronic liver failure. Clin Res Hepatol Gastroenterol, 2016, 40:41-50.
[2] Silva RDS, lombardi APG, Deborah Simão de Souza, et al. Activation of estrogen receptor beta (ERβ) regulates the expression of N-cadherin, E-cadherin and β-catenin in androgen-independent prostate cancer cells. Int J Biochem Cell Biol, 2018, 96:40-50.
[3] Wang S, Yan Y, Cheng Z, et al. Sotetsuflavone suppresses invasion and metastasis in non-small-cell lung cancer A549 cells by reversing EMT via the TNF-α/NF-κB and PI3K/AKT signaling pathway. Cell Death Discov, 2018, 4:26-41.
[4] Rosner M, Hengstschläger M. letter to the editor: human pluripotent stem cells release oncogenic soluble E-cadherin. Stem Cells, 2016, 34:2443-2446.
[5] Giovannini C, Minguzzi M, Genovese F, et al. Molecular and proteomic insight into Notch1 characterization in hepatocellular carcinoma. Oncotarget, 2016, 7:39609-39626.
[6] 中华医学会肝病学分会,中华医学会感染病学分会.慢性乙型肝炎防治指南(2015 年更新版).临床肝胆病杂志,2015,31:1941-1960.
[7] Cheng SF, Jiang YH, Kuo HC. Urothelial dysfunction and chronic inflammation are associated with increased bladder sensation in patients with chronic renal insufficiency. Int Neurourol J, 2018, 22:46-54.
[8] 黄坚, 沈佳庆, 汤海波, 等. sE-Cadherin早期评价急性胰腺炎严重程度的临床价值. 中国医科大学学报, 2014, 43:870-873.
[9] Qiao J, Qi K, Chu P, et al. Infusion of endothelial progenitor cells ameliorates liver injury in mice after haematopoietic stem cell transplantation. Liver Int, 2015, 35:2611-2620.
[10] 黄丹, 王彦, 张福杰. 北京地区HIV/HBV合并感染者与HBV单独感染者中HBV基因型分布. 中国艾滋病性病, 2019,25:227-230.
[11] Huang H, Wang J, Li W, et al. Serum HBV DNA plus RNA shows superiority in reflecting the activity of intrahepatic cccDNA in treatment-naïve HBV-infected individuals. J Clin Virol, 2018,18: 99-100.
[12] Zhong L , Shu W , Dai W , et al. Reactive oxygen species-mediated c-Jun NH2-terminal kinase activation contributes to hepatitis B virus X protein-induced autophagy via regulation of the Beclin-1/Bcl-2 interaction. J Virol, 2017, 91:1-17.
[13] Ruban VV, Archana PT , Sundararajan M , et al. Inflammation and oxidative stress in corneal tissue in experimental keratitis due to fusarium solani: amelioration following topical therapy with voriconazole and epigallocatechin gallate. Mycoses, 2018, 61:159-171.
[14] Emmerson A, Trevelin SC, Mongue-Din H, et al. Nox2 in regulatory T cells promotes angiotensin II-induced cardiovascular remodeling. J Clin Invest, 2018, 128:3088-3101.

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