Chinese Hepatolgy ›› 2021, Vol. 26 ›› Issue (6): 670-673.

• Other Liver Diseases • Previous Articles     Next Articles

The value of serum miR-221 and prolactin levels in the early diagnosis of non-alcoholic fatty liver disease in the elderly

TANG Gai-xiang1, LI Ya1, ZHANG Xu2   

  1. 1. Department of Gastroenterology, the First Affiliated Hospital of Zhengzhou University;
    2. Department of Laboratory Biochemistry, PLA General Hospital, Beijing 100853, China
  • Received:2020-06-28 Online:2021-06-30 Published:2021-07-19
  • Contact: LI Ya,Email:1377101812@qq.com

PDF (PC)

309

Abstract: Objective To investigate the value of serum miR-221 and prolactin levels in the early diagnosis of elderly non-alcoholic fatty liver disease.Methods We recruited 65 elderly patients diagnosed with nonalcoholic fatty liver disease (NAFLD) by liver biopsy from January 2019 to December 2019 in our hospital, including 40 patients in the simple non-alcoholic fatty liver (SNAFL) 25 cases in the group and non-alcoholic steatohepatitis (NASH) group and recruited 30 healthy elderly who applied for physical examination as control. We quantified the relative expression level and PRL level of miR-221 in the serum of the research subjects, and analyze the diagnostic efficacy through the receiver operating characteristic curve. Results The relative expression levels of miR-221 were from high to low in NASH group, SNAFL group, and control group. The P value of each group was <0.001. From high to low, the PRL levels were in control group, SNAFL group, NASH group (all P<0.05). The AUC values of miR-221 and PRL for the diagnosis of NAFLD were 0.795 and 0.880, respectively, and the P values were both <0.001. The sensitivity, specificity, positive predictive value, negative predictive value, and Youden index of PRL for the diagnosis of NAFLD were 83.3%, 87.7%, 91.9%, 75.8%, and 0.71, respectively, and the diagnostic efficacy was better than miR-221. The AUC values of miR-221 and PRL for the diagnosis of SNAFL and NASH were 0.862 and 0.710 respectively, and the P values were both <0.001. The sensitivity, specificity, positive predictive value, negative predictive value, and Yoden index of miR-221 for the diagnosis of SNAFL and NASH were 76.0%, 85.0%, 85.0%, 76.0%, and 0.61, respectively, and the diagnostic efficacy was better than PRL.Conclusion Serum miR-221 and prolactin levels have good diagnostic efficacy for elderly patients with NAFLD, and prolactin is superior to miR-221 in distinguishing patients with NAFLD from normal people, and miR-221 is superior to prolactin in distinguishing SNAFL from NASH patients.

Key words: microRNA, Prolactin, Elderly, Non-alcoholic fatty liver disease, Diagnostic test