Abstract: Objective To investigate the genotypic features of entecavir-resistant mutation in patients with chronic hepatitis B (CHB) in our hospital, and provide clinical data about characteristics and trends of entecavir-resistant mutation. Methods A total of 786 patients with entecavir-resistant mutation admitted to our hospital from January 2009 to October 2020 were selected. The drug-resistant situation were further analyzed. Results Among the 786 entecavir-resistant mutation patients, the numbers of hepatitis B virus (HBV) C genotype, HBV B genotype and HBV genotype were 705 (89.7%), 79 (10.1%) and 2 (0.3%), respectively. Twenty-one drug-resistant mutation types were found. The numbers of rtL180M + rtM204V (or rtM204I) + rtV173, rtL180M + rtM204V (or rtM204I) + rtT184, rtL180M + rtM204V + rtS202, rtL180M + rtM204V + rtS202 + rtT184, rtL180M + rtM204V + rtM250 mutation types were 219 (27.9%), 218 (27.7%), 175 (22.3%), 52 (6.6%) and 44 (5.6%) respectively. There were other 16 mutation types with a total number of 78 (9.9%). Conclusion HBV genotype C are the most common genotype among patients with entecavir-resistant mutation in our hospital. The rtL180M + rtM204V (or rtM204I) + rtV173 is the most prevalent mutation type and rtT184 is the most prevalent mutation site. Our research can help people understand the HBVgenotypes and mutation types of CHB patients with entecavir-resistant mutation in our hospital.

Key words: Chronic hepatitis B, Entecavir, Genotype, Drug-resistant mutation