The mechanism of lncRNA TSIX inhibiting proliferation and invasion of hepatocellular carcinoma cell by targeting miR-548-a-3p

Abstract

Abstract: Objective To investigate the mechanism of lncRNA TSIX inhibiting proliferation and invasion of hepatocellular carcinoma cell by targeting miR-548-a-3p. Methods The liver cancer cell line Huh7 group, lncRNA TSIX mimics group, and lncRNA TSIX inhibitor group were set up. After 48 hours of cell culturing in each group, the cell viability was measured by MTT method, and the number of single clones formed by crystal violet staining was measured, and the apoptosis level was measured by flow cytometry. The cell invasion level was measured by transwell chamber, and the lncRNA TSIX and miR-548-a-3p levels were measured by RT-PCR method. Results The OD value, survival rate, number of colonies formed, and number of membrane penetration in the lncRNA TSIX mimics group were lower than those in the Huh7 group (t=8.725, 5.437, 120.834, 64.321, P<0.05), and the OD value, survival rate, number of colonies formed, number of membrane penetration in the lncRNA TSIX inhibitor group were higher than those in the Huh7 group and lncRNA TSIX inhibitor group (t=4.124, 3.784, 10.462, 7.965, 37.192, 174.632, 30.703, 104.562, P<0.05). The apoptotic rate and lncRNA TSIX in the lncRNA TSIX mimics group was higher than those in the Huh7 group [(18.52±2.45) vs (15.63±2.13), (4.47±0.22) vs (2.89±0.21)] (t=10.531, 12.372, P<0.05), and the apoptotic rate and lncRNA TSIX in the lncRNA TSIX inhibitor group was lower than those in the Huh7 group and the lncRNA TSIX inhibitor group [(8.14±2.45) vs (15.63±2.13), (18.52±2.45); (1.58±0.25) vs (2.89±0.21), (4.47±0.22)] (t=37.385, 48.021, 30.703, 104.562, P<0.05). The miR-548-a-3p in lncRNA TSIX mimics group was lower than that in Huh7 cell group [(2.39±0.19) vs (4.87±0.20)] (t=11.265, P<0.05), and miR-548-a-3p in lncRNA TSIX inhibitor group was higher than that in Huh7 group and lncRNA TSIX inhibitor group [(6.32±0.19) vs (4.87±0.20), (2.39±0.19)] (t=6.612, 10.058, P<0.05). Conclusion LncRNA Tsix significantly inhibited the proliferation and invasion of HCC cells and promoted the apoptosis of HCC cells. The mechanism may be related to the negative regulation of miR-548-a-3p by lncRNA Tsix.

Key words: LncRNA TSIX, MiR-548-a-3p, Hepatocellular carcinoma, Cell proliferation, Cell invasion

WANG Jia-le, LI Cheng, LI Guang-ming. The mechanism of lncRNA TSIX inhibiting proliferation and invasion of hepatocellular carcinoma cell by targeting miR-548-a-3p[J]. Chinese Hepatolgy, 2022, 27(2): 213-217.

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