The association between SHP2 expression and hepatic stellate cells apoptosis in rat liver tissues in vivo during the pathologic process of hepatic fibrogenesis

Abstract

Abstract: Objective To investigate the association between SHP2 expression and hepatic stellate cells (HSC) apoptosis in rats liver tissues in vivo during the pathologic process of hepatic fibrogenesis.Methods 50 healthy male SD rats were randomly divided into a control group (n=10) and a model group (n=40). Rat model of hepatic fibrogenesis was established by intraperitoneal injection of carbon tetrachloride (CCl4). Masson's trichrome and Hematoxylin and eosin (HE) staining were used for the histological analysis of the changes in liver tissues. The expression of SHP2 in hepatic tissues was analyzed by immunohistochemical staining. Dual staining of alpha-smooth muscle action (α-SMA) for HSC and TUNEL for apoptotic cells was used for analyzing the apoptotic indexes of HSC in the fibrogenic liver tissues.Results By immunohistochemical staining, it was demonstrated that the integral optical density (IOD) of SHP2 (0.19±0.01) in the liver tissues of the control group was lower than those of 0.23±0.01, 0.27±0.01, 0.30±0.01, 0.33±0.01 in the fibrogenic liver tissues of the model groups collected at 2, 4, 6, 8 weeks post CCl4 administration, with a notably increasing trend (P<0.05). Dual staining of α-SMA and TUNEL demonstrated that there were few apoptotic HSC in liver tissues of the control group, whereas both of the apoptotic HSC and the activated HSC coexisted in fibrogenic liver tissues. The apoptotic indexes of HSC in rats liver tissues at 2, 4, 6, and 8 weeks post CCl4 administration were 47%±1%, 41%±2%, 35%±1% and 29%±1%, respectively, showing a gradually lowering trend (P<0.05).Conclusion During the pathological process of rat liver fibrogenesis, there is an negative correlation between the expression level of SHP2 and the HSC apoptosis in liver tissues in vivo.

Key words: SHP2, Hepatic fibrosis, Hepatic stellate cells, Apoptosis

HAO Li-sen, YANG Xiao-shi, ZHANG Peng-lei, CHEN Pan-pan, ZHAN Zong-yuan, HE Yu, MIAO Xiao-jia. The association between SHP2 expression and hepatic stellate cells apoptosis in rat liver tissues in vivo during the pathologic process of hepatic fibrogenesis[J]. Chinese Hepatolgy, 2022, 27(4): 451-454.

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