The changes of histone acetylation modification in low invasive hepatocellular carcinoma cells

Abstract

Abstract: Objective To investigate the changes of histone acetylation modification in normal hepatocyte line LO2 cells and low invasive hepatocellular carcinoma (HCC) line MHCC-97L cells.Methods The proliferation and apoptosis of LO2 and MHCC-97L cells were detected by real-time cell analysis (RTCA); the modification of acH3K14, acH3K18, acH3K27 in LO2 and MHCC-97L cells were analyzed by Western Blot.Results The results of RTCA showed that the proliferation rate of low invasive MHCC-97L cells was significantly higher than that of LO2 cells, of which the difference was statistically significant (P<0.05). There was little difference in apoptosis between the two cell lines under fluorescence microscope. The levels of acetylation modification of histone H3K14, H3K18, H3K27 in MHCC-97L cells were significantly lower than those in LO2 cells (P<0.05).Conclusion Compared to LO2 cells, the multiplication rate of MHCC-97L cells was significantly higher whereas the natural apoptosis rate was not different. The histone modification of acH3K14, acH3K18 and acH3K27 in MHCC-97L cells were decreased which might be associated with the hepatocarcinogenesis. Monitoring the levels of histone acetylation in hepatocytes may have a reference value for the clinical diagnosis of HCC and the upregulation of it may be a target for drug development against HCC.

Key words: Low invasive hepatocellular carcinoma cells, Proliferation, Apoptosis, Histone Acetylation

ZHANG Jian-dong, LI Liang-he, CHENG Hai-yu, JIANG Dong-bin, YANG Qin, ZHAN Wei. The changes of histone acetylation modification in low invasive hepatocellular carcinoma cells[J]. Chinese Hepatolgy, 2022, 27(12): 1296-1299.

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