Predictive value of lncRNAs expression level in peripheral blood before chemotherapy on anti-tuberculosis drug induced liver iniury

Abstract

Abstract: Objective To study the predictive value of long non-coding RNAs (lncrnas) expression in peripheral blood before chemotherapy on anti-tuberculosis drug induced liver iniury(ATDILI). Methods From May 2017 to may 2019, patients with newly diagnosed pulmonary tuberculosis in our hospital were selected, the expression level of lncRNAs in peripheral blood before chemotherapy were determined by lncRNAs microarray and fluorescence quantitative PCR. The patients were divided into ATDILI group and non-ATDILI group according to whether ATDILI occured during chemotherapy. ROC curve was used to analyze the predictive value of lncRNAs for ATDILI, and logistic regression model was used to analyze the influencing factors of ATDILI. Results By lncrnas microarray analysis, compared with non-ATDILI group, the expression of 46 lncRNAs in peripheral blood of ATDILI group significantly up-regulated and the 5 most upregulation lncRNAs were lncRNA MALAT1, HNF1α-AS1, H19, XIST and CCAT1. By fluorescence quantitative PCR, the expression of lncRNA MALAT1, HNF1α-AS1, XIST and CCAT1 in peripheral blood of ATDILI group were significantly higher than non-ATDILI group; ROC curve analysis showed that lncRNA MALAT1, HNF1α-AS1, XIST and CCAT1 had predictive value for ATDILI; logistic regression analysis showed that gender, malnutrition, viral hepatitis history, drinking history and lncRNA MALAT1, XIST were the influencing factors of ATDILI. Conclusion The increased expression of lncRNA MALAT1 and XIST in peripheral blood before chemotherapy relates to the occurrence of ATDILI, which can be used as a predictor for ATDILI.

Key words: Antituberculosis drugs, Liver injury, Long non-coding RNA, Prediction, Influencing factors

LI Xiang, GAO Jing, BAO Hai-yang, ZHU Yan-qiu, CHEN Yan-hui, WANG Xing-hua. Predictive value of lncRNAs expression level in peripheral blood before chemotherapy on anti-tuberculosis drug induced liver iniury[J]. Chinese Hepatolgy, 2023, 28(3): 346-350.

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