Alteration of T-cell subsets in peripheral blood and immunologic function in liver cirrhosis patients with splenectomy
Guo Ting-ting
2016, 21(9):
726-728.
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Objective To investigate the alteration of peripheral blood T lymphocyte subsets and varied immune function in liver cirrhosis patients after splenectomy. Methods The absolute quantity of peripheral blood T lymphocyte subsets was detected using flow cytometry in 75 patients with liver cirrhosis before and after splenectomy. Results Compared with the prior to splenectomy, obviously decrease in absolute value of T cell was received in patients after splenectomy. The percentages of CD3+, CD4+ and CD8+ T cells were decreased from (63.47±6.45)%, (33.08±3.05)%, (22.45±7.85)% to (48.62±5.85)%, (26.28±3.87)%, (15.89±4.84)% respectively. However, CD4+/CD8+ ratio rose dramatically from (1.47±0.43) to (1.69±0.53) (t=P<0.05). The percentage of patients, which the frequencies of CD3+, CD4+ and CD8+ T cells were less than 50%, 27%, and 15% respectively, was increased from 8%, 20% and 14.7% to 37.3%, 50.7% and 32%, respectively. Additionaly, the percentage of patients with CD4/CD8<0.71 increased from 5.3% to 6.7%. Compared to before splenectomy, abnormal percentage of lymphocyte subsets after splenectomy, including CD3+、CD4+ and CD8+, was increased significantly (t=3.59, 6.44 and 4.89 respectively, all P<0.01), meanwhile the levels of cytokines secreted by T cells after splenectomy, including IL-2, IL-4, IFN-γ and TNF-a, were decreased significantly. Conclusion There might be immune disorders in liver cirrhosis patients after splenectomy, such as apparent reduction in quantity of T lymphocyte subsets and hyperfunction of CD4+ T cell compared to CD8+ T cell. As the disease condition aggravates, the immune response ability of CD4+ T cell could be further weaken. Patients after splenectomy showing significant abnormality of lymphocyte subsets should be alert to the occurrence of tumor or infection.
